• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:

    公开号:SU1834646A3
    申请号:SU884356128
    申请日:1988-07-20
    公开日:1993-08-15
    发明作者:Dzhiovanetto Rodzher
    申请人:Dzhiovanetto Rodzher；
    IPC主号:
    专利说明:

    The invention relates to a method for isolating a sweet substance from plants, in particular, to a method for isolating steviosides from dried plant material.
    The aim of the invention is to increase the degree of purity obtained from Stevia rebuadiana Bertoni product.
    This goal is achieved by the proposed method for the isolation of steviosides by extracting dried plant materials Stevia rebuadiana Bertoni with water, treating the extract with strongly acid cation exchange resin and treating with slightly alkaline ion exchanger and drying the eluate, which consists in. that before the treatment with strongly acidic cation exchange resin, the extract is treated with calcium hydroxide at a pH of 8.3 - 11.0, followed by removal of the precipitate formed, and the eluate is filtered before drying.
    It is advisable to concentrate before filtering and repeat the treatment with ion exchangers 1-5 times.
    After each treatment cycle of the ion exchange resin, filtration and concentration can be carried out.
    Treatment of the extract with calcium hydroxide is carried out at room temperature and stirring for several hours, for example 20 hours
    Example 1.1 kg of dried plant material Stevia rebuadiana Bertoni is extracted with water taken in an amount of 10 l at room temperature. The resulting extract is treated with calcium hydroxide at room temperature and a pH of 8.5 with stirring for 20 hours and then filtered using a fine filter system to remove the precipitate formed. After removing excess water, the resulting clear solution is treated with Dowex 50 W, followed by elution with distilled water, and the resulting eluate is subjected to treatment with weakly alkaline Dowex WGR ion exchanger followed by elution with distilled water. The resulting eluate is subjected to fine filtration. Then the specified cycle of treatment with ion exchangers, which is carried out at room temperature and atmospheric pressure, is repeated three times.
    1 834 646 AZ
    After elution and filtration at the end of the last treatment with ion exchangers, the resulting eluate is concentrated and subjected to fine filtration. After drying, 107 g of product containing 75% pure steviosides are obtained,
    HT r memer 2. 1 kg of dried plant material Stevia rebuadiana Bertoni is extracted with water taken in an amount of 10 l. at room temperature. The extract obtained is treated with calcium hydroxide at room temperature and a pH of 9.5 with stirring for 18 hours and then filtered using a fine filter system in order to remove the precipitate formed. After removal of excess water, the resulting clear solution was subjected to treatment with highly acidic Dowex 88 ion exchanger at room temperature and atmospheric pressure, followed by elution with distilled water, and the resulting eluate was subjected to treatment with weakly alkaline Dowex 66 ion exchanger at room temperature and atmospheric pressure, followed by elution with distilled water. The resulting eluate is subjected to fine filtration and concentrated to dryness. Obtain 140 g of a product containing 70% pure steviosides.
    Example 3. 1 kg of dried plant material Stevia rebuadiana Bertoni is extracted with water taken in an amount of 20 l at room temperature. The resulting extract was treated with calcium hydroxide at room temperature and a pH of 11.0 with stirring for 15 hours and then filtered using a fine filter system. to remove the precipitate. After removal of excess water, the resulting clear solution was subjected to treatment with Dowex 88 strongly acidic ion exchanger, followed by elution with distilled water, and the resulting eluate was subjected to treatment with Dowex 66 weakly alkaline ion exchanger, followed by elution with distilled water. The resulting eluate is successively passed through a layer of Dowex 88 ion exchange resin and a Dowex 66 ion exchange resin layer. Then, the indicated treatment with ion exchangers, which is carried out at room temperature and atmospheric pressure, is repeated two times. After elution and fine filtration at the end of the last treatment with ion exchangers, the resulting eluate is concentrated to dryness. Obtain 88 g of a product containing 95% pure steviosides.
    Example 4. Repeat example 1 with the difference that the cycle of treatment with ion exchangers is repeated 5 times. This gives 102 g of a product containing 80% pure steviosides.
    权利要求:
    Claims (4)
    [1]
    Claim
    1. A method of isolating steviosides by extracting dried plant material Stevia rebuadiana Bertoni with water, treating the extract with strongly acidic cation exchange resin and weakly alkaline ion exchange resin and drying the eluate, characterized in that, in order to increase the purity, the extract is treated with calcium hydroxide at pH 8.3 -11.0 followed by removal of the precipitate, and before drying, the eluate is subjected to filtration.
    [2]
    2. The method according to claim 1, characterized in that prior to filtration, concentration is carried out.
    [3]
    3. The method according to claim 1, characterized in that the treatment with ion exchangers is repeated 1-5 times.
    [4]
    4. The method according to PP. 1 and 3, characterized in that. that after each treatment cycle with ion exchangers, filtration and concentration are carried out.
    类似技术:
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    同族专利:
    公开号 | 公开日
    ES2046979T3|1994-02-16|
    EP0302948B1|1993-12-01|
    IE882213L|1989-01-21|
    CN1021826C|1993-08-18|
    NO883234D0|1988-07-20|
    NO883234L|1989-02-13|
    CN1030762A|1989-02-01|
    DD273442A5|1989-11-15|
    NZ225471A|1991-02-26|
    JPH01131191A|1989-05-24|
    NO173913C|1994-02-23|
    AU1921988A|1989-01-27|
    CA1310963C|1992-12-01|
    DK406188A|1989-01-22|
    ZA885176B|1989-04-26|
    NO173913B|1993-11-15|
    IN171592B|1992-11-21|
    DE3788351D1|1994-01-13|
    DK406188D0|1988-07-20|
    IE63706B1|1995-05-31|
    EP0302948A3|1989-03-22|
    AU609154B2|1991-04-26|
    EP0302948A2|1989-02-15|
    US4892938A|1990-01-09|
    AT97910T|1993-12-15|
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    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    EP87110541A|EP0302948B1|1987-07-21|1987-07-21|Process for obtaining steviosides from plants|
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